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Assessment of the Antimicrobial and Antibiofilm Activities of Trigonella smyrnea Boiss. Growing in Türkiye
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Abstract
Background: The aim of this study is to assess the antimicrobial and antibiofilm capabilities of Trigonella smyrnea Boiss. plant species.
Methods: The antimicrobial activity of an ethanol-derived extract from the aerial parts, seeds and seed coat of Trigonella smyrnea were tested using the microdilution technique against 5 reference bacteria and three yeast strains. Additionally, the ability of the extracts to inhibit both biofilm formation and pre-formed biofilms produced by Pseudomonas aeruginosa was assessed using the crystal violet method.
Results: The utilized samples exhibited mild to moderate antimicrobial activity against the examined bacteria and yeasts, with minimum inhibitory concentration (MIC) values ranging from 250 to 62.5 µg/mL. The aerial parts, seed and seed coat extracts inhibited biofilm formation by approximately 36%, 28%, 40% and 30%, 25%, 33% at MIC/2 and MIC/4 MIC, respectively. In addition, preformed biofilm inhibition rates of aerial parts, seed and seed coat extracts were MIC/2: 11%, 26%, 26%; MIC: 15%,
30%, 31% and in 2×MIC: 31%, 38%, 38%, respectively.
Conclusion: Our data indicates that the extracts have mild to limited antimicrobial activity and moderate potential in combating biofilm formation. Especially when compared to seed extract, aerial parts and seed coat extracts showed better antibiofilm activity. In conclusion, although extracts of this species are promising in terms of antibiofilm activity, these findings should be supported by further study.
Cite this article as: Uras Güngör ŞS, Öksüz Z, Assessment of the antimicrobial and antibiofilm activities of Trigonella smyrnea boiss. growing in Türkiye. Trends Pharm. 2024, 1, 13, doi: 10.5152/TrendsPharm.2024.23013
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